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biotin-labeled rabbit anti-human bdnf polyclonal antibodies  (PeproTech)

 
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    PeproTech biotin-labeled rabbit anti-human bdnf polyclonal antibodies
    Biotin Labeled Rabbit Anti Human Bdnf Polyclonal Antibodies, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin-labeled rabbit anti-human bdnf polyclonal antibodies/product/PeproTech
    Average 90 stars, based on 1 article reviews
    biotin-labeled rabbit anti-human bdnf polyclonal antibodies - by Bioz Stars, 2026-03
    90/100 stars

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    Effect of prenatal maternal stress (PMS) regulation of <t>Bdnf</t> splice variant and total Bdnf expression in the F1 and F2 generations. Quantitative real–time PCR analysis showed that PMS/oxytocin mediated alterations in the expression of Bdnf exon—III, IV and exon—VI transcripts. The analysis showed that PMS differentially altered the expression, level of exon-III increased, and exon-IV and VI decreased in (A) F1 (PMS) and (D) F2 (PMS) offspring. Administration of oxytocin minimized the PMS-induced effect, decreased exon-III, and increased exon-IV levels in (A) F1 (PMS+OXT) and (D) F2 (PMS+OXT) offspring. Furthermore, the level of total Bdnf was decreased by PMS in the PMS F1 and PMS F2 offspring, and oxytocin administration reverse the PMS induced effect. Correlation analysis showed that the level of total Bdnf mRNA significantly associated reference (B,E) , working memory (C,F) in F1 and F2 offspring. Data are represented as mean ± SE, and statistical significance is indicated by ** p < 0.01; *** p < 0.001; NS, not significant.
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    biotin-labeled rabbit anti-human bdnf polyclonal antibodies  (PeproTech)
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    PeproTech biotin-labeled rabbit anti-human bdnf polyclonal antibodies
    Effect of prenatal maternal stress (PMS) regulation of <t>Bdnf</t> splice variant and total Bdnf expression in the F1 and F2 generations. Quantitative real–time PCR analysis showed that PMS/oxytocin mediated alterations in the expression of Bdnf exon—III, IV and exon—VI transcripts. The analysis showed that PMS differentially altered the expression, level of exon-III increased, and exon-IV and VI decreased in (A) F1 (PMS) and (D) F2 (PMS) offspring. Administration of oxytocin minimized the PMS-induced effect, decreased exon-III, and increased exon-IV levels in (A) F1 (PMS+OXT) and (D) F2 (PMS+OXT) offspring. Furthermore, the level of total Bdnf was decreased by PMS in the PMS F1 and PMS F2 offspring, and oxytocin administration reverse the PMS induced effect. Correlation analysis showed that the level of total Bdnf mRNA significantly associated reference (B,E) , working memory (C,F) in F1 and F2 offspring. Data are represented as mean ± SE, and statistical significance is indicated by ** p < 0.01; *** p < 0.001; NS, not significant.
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    Effect of prenatal maternal stress (PMS) regulation of Bdnf splice variant and total Bdnf expression in the F1 and F2 generations. Quantitative real–time PCR analysis showed that PMS/oxytocin mediated alterations in the expression of Bdnf exon—III, IV and exon—VI transcripts. The analysis showed that PMS differentially altered the expression, level of exon-III increased, and exon-IV and VI decreased in (A) F1 (PMS) and (D) F2 (PMS) offspring. Administration of oxytocin minimized the PMS-induced effect, decreased exon-III, and increased exon-IV levels in (A) F1 (PMS+OXT) and (D) F2 (PMS+OXT) offspring. Furthermore, the level of total Bdnf was decreased by PMS in the PMS F1 and PMS F2 offspring, and oxytocin administration reverse the PMS induced effect. Correlation analysis showed that the level of total Bdnf mRNA significantly associated reference (B,E) , working memory (C,F) in F1 and F2 offspring. Data are represented as mean ± SE, and statistical significance is indicated by ** p < 0.01; *** p < 0.001; NS, not significant.

    Journal: Frontiers in Neuroscience

    Article Title: Prenatal maternal life adversity impacts on learning and memory in offspring: implication to transgenerational epigenetic inheritance

    doi: 10.3389/fnins.2025.1518046

    Figure Lengend Snippet: Effect of prenatal maternal stress (PMS) regulation of Bdnf splice variant and total Bdnf expression in the F1 and F2 generations. Quantitative real–time PCR analysis showed that PMS/oxytocin mediated alterations in the expression of Bdnf exon—III, IV and exon—VI transcripts. The analysis showed that PMS differentially altered the expression, level of exon-III increased, and exon-IV and VI decreased in (A) F1 (PMS) and (D) F2 (PMS) offspring. Administration of oxytocin minimized the PMS-induced effect, decreased exon-III, and increased exon-IV levels in (A) F1 (PMS+OXT) and (D) F2 (PMS+OXT) offspring. Furthermore, the level of total Bdnf was decreased by PMS in the PMS F1 and PMS F2 offspring, and oxytocin administration reverse the PMS induced effect. Correlation analysis showed that the level of total Bdnf mRNA significantly associated reference (B,E) , working memory (C,F) in F1 and F2 offspring. Data are represented as mean ± SE, and statistical significance is indicated by ** p < 0.01; *** p < 0.001; NS, not significant.

    Article Snippet: Membranes were gently washed with TBS-T (5 min/wash) and then incubated in any one of the following primary antibodies [anti-histone H3 rabbit monoclonal antibody (Cat# 4499, 1:5,000; Cell Signalling Technology, Inc.); anti-H3K4me2 rabbit monoclonal antibody (Cat# 9725, 1:5,000; Cell Signalling Technology, Inc.); antiH3K4me3 rabbit monoclonal antibody (Cat# 9725, 1:5,000; Cell Signalling Technology, Inc.); anti-pro-BDNF rabbit polyclonal antibody (Cat# SC-546, 1:2,000; Santa Cruz Biotechnology), mature BDNF rabbit polyclonal antibody (Cat# PA5-85730, 1: 2,000; Invitrogen), and antiß-actin rabbit polyclonal antibody (Cat# SC-47778, 1:2,000; Santa Cruz Biotechnology, Inc.) for about 12–16 h at 4°C.

    Techniques: Variant Assay, Expressing, Real-time Polymerase Chain Reaction

    Effect of prenatal maternal stress (PMS) alter the level of pro-BDNF and mature BDNF in the F1 and F2 generations. (A) Representative western blots showing the expression levels of pro-and mature BDNF in the F1 and F2. The analysis showed that PMS significantly reduced the level of pro-and mature BDNF in F1 offspring, which is positively correlated with reference (B,D) , working memory (C,E) in F1 offspring. Administration of oxytocin minimized the PMS-induced effect, restored the level of pro-and mature BDNF in F1 (PMS+OXT) and F2 (PMS+OXT) offspring.

    Journal: Frontiers in Neuroscience

    Article Title: Prenatal maternal life adversity impacts on learning and memory in offspring: implication to transgenerational epigenetic inheritance

    doi: 10.3389/fnins.2025.1518046

    Figure Lengend Snippet: Effect of prenatal maternal stress (PMS) alter the level of pro-BDNF and mature BDNF in the F1 and F2 generations. (A) Representative western blots showing the expression levels of pro-and mature BDNF in the F1 and F2. The analysis showed that PMS significantly reduced the level of pro-and mature BDNF in F1 offspring, which is positively correlated with reference (B,D) , working memory (C,E) in F1 offspring. Administration of oxytocin minimized the PMS-induced effect, restored the level of pro-and mature BDNF in F1 (PMS+OXT) and F2 (PMS+OXT) offspring.

    Article Snippet: Membranes were gently washed with TBS-T (5 min/wash) and then incubated in any one of the following primary antibodies [anti-histone H3 rabbit monoclonal antibody (Cat# 4499, 1:5,000; Cell Signalling Technology, Inc.); anti-H3K4me2 rabbit monoclonal antibody (Cat# 9725, 1:5,000; Cell Signalling Technology, Inc.); antiH3K4me3 rabbit monoclonal antibody (Cat# 9725, 1:5,000; Cell Signalling Technology, Inc.); anti-pro-BDNF rabbit polyclonal antibody (Cat# SC-546, 1:2,000; Santa Cruz Biotechnology), mature BDNF rabbit polyclonal antibody (Cat# PA5-85730, 1: 2,000; Invitrogen), and antiß-actin rabbit polyclonal antibody (Cat# SC-47778, 1:2,000; Santa Cruz Biotechnology, Inc.) for about 12–16 h at 4°C.

    Techniques: Western Blot, Expressing

    Effect of prenatal maternal stress (PMS) alter the level of pro-BDNF and mature BDNF in the F1 and F2 generations. (A) The analysis showed that PMS significantly reduced the level of pro-and mature BDNF in F2 offspring, which is positively correlated with reference (B,D) , working memory (C,E) in F2 offspring. Administration of oxytocin minimized the PMS-induced effect, restored the level of pro-and mature BDNF in F1 (PMS+OXT) and F2 (PMS+OXT) offspring.

    Journal: Frontiers in Neuroscience

    Article Title: Prenatal maternal life adversity impacts on learning and memory in offspring: implication to transgenerational epigenetic inheritance

    doi: 10.3389/fnins.2025.1518046

    Figure Lengend Snippet: Effect of prenatal maternal stress (PMS) alter the level of pro-BDNF and mature BDNF in the F1 and F2 generations. (A) The analysis showed that PMS significantly reduced the level of pro-and mature BDNF in F2 offspring, which is positively correlated with reference (B,D) , working memory (C,E) in F2 offspring. Administration of oxytocin minimized the PMS-induced effect, restored the level of pro-and mature BDNF in F1 (PMS+OXT) and F2 (PMS+OXT) offspring.

    Article Snippet: Membranes were gently washed with TBS-T (5 min/wash) and then incubated in any one of the following primary antibodies [anti-histone H3 rabbit monoclonal antibody (Cat# 4499, 1:5,000; Cell Signalling Technology, Inc.); anti-H3K4me2 rabbit monoclonal antibody (Cat# 9725, 1:5,000; Cell Signalling Technology, Inc.); antiH3K4me3 rabbit monoclonal antibody (Cat# 9725, 1:5,000; Cell Signalling Technology, Inc.); anti-pro-BDNF rabbit polyclonal antibody (Cat# SC-546, 1:2,000; Santa Cruz Biotechnology), mature BDNF rabbit polyclonal antibody (Cat# PA5-85730, 1: 2,000; Invitrogen), and antiß-actin rabbit polyclonal antibody (Cat# SC-47778, 1:2,000; Santa Cruz Biotechnology, Inc.) for about 12–16 h at 4°C.

    Techniques: